Establishment of Fruit Bat Cells (Rousettus aegyptiacus) as a Model System for the Investigation of Filoviral Infection
Figure 2
Expression of viral proteins in different cell lines.
(A) 4×105 HUH7, VeroE6 or R06E cells were infected with 0.1 TCID50/cell MARV or ZEBOV. Cell lysates were prepared at 48 and 72 h p.i., subjected to Western blot analysis to detect cellular tubulin and VP40 of MARV and ZEBOV using mouse monoclonal antibodies. The total amount of cellular proteins in the samples was quantified by separating cell lysates on SDS PAGE, which were then stained with Coomassie Blue. Using the Odyssey Infrared Imaging Application Software, the protein signals were quantified. VP40 levels normalized to total cell protein are shown in (B).