Antiangiogenic and Antitumor Effects of Trypanosoma cruzi Calreticulin
Figure 2
TcCRT and N-TcCRT inhibit capillary morphogenesis.
Phase contrast images of HUVECs organization in the Matrigel morphogenesis assay are shown. Cells were cultured on the surface of Matrigel and incubated with: A and F. PBS (control), B. HuCRT, C. TcCRT, D. N-TcCRT and G. R-TcCRT, all of them at 1µM, for 6 h at 37°C, 5% CO2. E and H. Tubular structures were quantified by counting at low power fields. Data are represented as means ± SEM, obtained from four fields. **, p<0.01. Results are representative of 3 independent experiments. Original magnification, ×10.