Electroporation Facilitates Introduction of Reporter Transgenes and Virions into Schistosome Eggs
Figure 3
Luciferase activity in Schistosoma mansoni eggs.
Detection of luciferase activity in extracts of eggs treated with capped mRNA encoding firefly luciferase (mFLuc). Panel A: luciferase activity three hours after soaking or electroporation with 130 ng/µl of mFLuc; a, negative controls soaked without mFLuc; b, eggs soaked with mFLuc; c, negative control eggs electroporated without mRNA; d, eggs electroporated with mFLuc. RLUs/µg, relative light units per microgram of egg protein. Panel B: luciferase activity three hours after electroporation of eggs in control (mock) and experimental groups with 100ng/µl and 130ng/µl of mFLuc, and at 30 hours after electroporation with 130 ng/µl (indicated with cross symbol). Asterisks denote statistically significant differences (P≤0.05) among groups.