The Hookworm Tissue Inhibitor of Metalloproteases (Ac-TMP-1) Modifies Dendritic Cell Function and Induces Generation of CD4 and CD8 Suppressor T Cells
Figure 2
Ac-TMP-1 treatment affects their phenotype and cytokine expression bone marrow-derived dendritic cells.
A. Flow cytometry profiles of CD80, CD86 and MHC Class I and II expression levels in CD11c+ bone marrow-derived DCs incubated for 16 hours with RPMI or 1–100 µg recombinant Ac-TMP-1. Data are expressed as average±SEM of 3 different experiments. *: Statistically significant, P = 0.02. B. Flow cytometry profiles of MHC Class II expression levels in DCs incubated at different time points with RPMI, 50 µg recombinant Ac-TMP-1 or 50 µg OVA. Data are expressed as average±SEM of 3 different experiments. *: Statistically significant, P = 0.008. C. Intracellular cytokine staining for IL-12p40/p70 and IL-10 in CD11c+ bone marrow-derived DCs unstimulated or stimulated with Ac-TMP-1 or OVA. Data are expressed as average±SEM of 4 different experiments. *: Statistically significant, P = 0.001. D. Secretion of TGF-Β in supernatants of DCs primed with the antigens as in B. Data are expressed as average±SEM of 3 different determinations. *: Statistically significant, P = 0.0001.