Influence of Ecto-Nucleoside Triphosphate Diphosphohydrolase Activity on Trypanosoma cruzi Infectivity and Virulence
Figure 5
Heterologous expression of T. cruzi Ecto-NTPDase-1 and the effect of ecto-ATPase inhibitors on purified protein.
A) SDS-PAGE stained with coomassie blue. A 15 µL sample of each step of production and purification of Ecto-NTPDase-1 was applied in each lane, showing the purified protein with approx. 66 kDa. MW, molecular weight markers. B) ATPDase activity of purified Ecto-NTPDase-1 in the presence of ecto-ATPase inhibitors. The results represent the percent activity with inhibitor related to the activities in the absence of inhibitors. Data are mean±SE of two independent experiments, each assayed in triplicate.