Use of Genomic DNA as an Indirect Reference for Identifying Gender-Associated Transcripts in Morphologically Identical, but Chromosomally Distinct, Schistosoma mansoni Cercariae
Figure 3
Increased number of gender-associated, differentially expressed transcripts are identified using S. mansoni gDNA as a common reference.
Venn diagram demonstrates numbers of differentially expressed gender-associated cercarial transcripts as revealed by utilization of two different DNA microarray hybridization strategies. A) represents an indirect method for calculating gene expression ratios where gender-associated transcripts were identified through a statistical Student's t-test comparison of male cDNA v reference gDNA (n = 3) and female cDNA v reference gDNA (n = 3). B) and C) both represent direct methods for calculating gene expression ratios as identified by analysis of five independent experiments of male cDNA v female cDNA targets in both dye combinations. B) denotes female-biased transcripts identified. C) represents male-biased transcripts. All differentially expressed transcripts identified here are listed in the Dataset S2. D) Lists of the concordant, differentially expressed male (188) and female (156) transcripts were analyzed by Gene Ontology classification and the percentages of transcripts defined by Molecular Function, Cellular Composition and Biological Process categories are illustrated.