Novel Triazine JPC-2067-B Inhibits Toxoplasma gondii In Vitro and In Vivo
Figure 2
Effect of JPC-2067-B on T. gondii in human foreskin fibroblasts.
A. Thymidine uptake assay demonstrates no toxic effect on host cells. B. Uracil uptake assay demonstrates that JPC-2067-B is effective against T. gondii at low nanomolar concentrations. C. Micrographs showing marked inhibition of T. gondii by JPC-2067-B. Note absence of plaques and parasites in treated cultures. Concentrations prepared are shown. D. Micrograph showing absence of destruction of monolayers infected, exposed to JPC-2067-B for 4 days and cultured for prolonged times with T. gondii. The control monolayer was completely destroyed by 5 days of culture. This contrasts with similarly infected monolayers in the micrograph exposed to JPC-2067-B for 4 days and then with the JPC-2067-B removed. No plaques or Toxoplasma were seen throughout 52 days of culture, demonstrating that the JPC-2067-B is cidal and no drug resistant mutants were selected in this experiment. No plaques were present from 4 days through the subsequent 1 and 1/2 months after in vitro challenge.