Down-Regulation of the Interferon Signaling Pathway in T Lymphocytes from Patients with Metastatic Melanoma
Figure 2
Percentage of STAT1-pY701-Positive Cells in Lymphocytes from Patients with Melanoma and Healthy Controls in Response to IFN Stimulation
PBMCs from patients with melanoma and healthy controls were stimulated with 1,000 IU/ml IFN-α, IFN-β, or IFN-γ. The percentage of STAT1-pY701-positive cells was measured by Phosflow. p-Values were calculated using the Wilcoxon rank sum test (one-sided). Medians are indicated by the bar in each scatter column. H, healthy; M, melanoma.
(A–D) IFN-α-stimulated PBMCs. Lymphocytes (A); CD8 T cells (B); CD4 T cells (C); B cells (D).
(E–H) IFN-β-stimulated PBMCs. Lymphocytes (E); CD8 T cells (F); (G) CD4 T cells (G); B cells (H).
(I–L) IFN-γ-stimulated PBMCs. Lymphocytes (I); CD8 T cells (J); CD4 T cells (K); B cells (L).