Gene Expression Signatures That Predict Radiation Exposure in Mice and Humans
Figure 1
Scheme for Development of Radiation Response Expression Signature
PB MNCs were collected from control (0 cGy), 50 cGy-irradiated, 200 cGy-irradiated, and 1,000 cGy-irradiated C57Bl6 mice. Gene array analyses were performed on RNA isolated from n = 7 replicates from each condition, and metagene profiles were developed to represent the four different levels of radiation exposure. A leave-one-out cross-validation study was performed, which revealed the highly predictive nature of these metagene profiles. Finally, these profiles were independently validated by blinded analysis of PB samples from human patients who had undergone TBI to determine the capacity for these profiles to predict human radiation exposure.