A New Arthritis Therapy with Oxidative Burst Inducers
Figure 1
Substances Inducing Oxidative Burst In Vitro Prevent Arthritis
(A) Structure of the oil is important for oxidative burst-inducing effect in vitro. Extracellular ROS production was measured with an isoluminol assay on HL-60 cells after in vitro stimulation with alkanes, phytol (Ph), or pristane (Pr). Data represent one of several experiments. #Value is divided by 10.
(B) Alkanes longer than C15 induce arthritis. Arthritogenicity of alkanes (n = 4), phytol (Ph; n = 5), and pristane (Pr; n = 7) after SC administration in DA.Ncf1DA rats. Results are presented as cumulative incidence in percent.
(C) Oxidative burst-inducing alkanes prevent arthritis. Arthritis-preventing effect of selected alkanes after SC administration day −5 before PIA induction (control, n = 17; C11, n = 6; and C16, n = 5) in DA.Ncf1DA rats.
(D) Phytol inhibits development of arthritis. Preventive effect of phytol on PIA in DA.Ncf1DA rats. Phytol was injected SC day −5 prior to pristane injection (control, n = 12; phytol, n = 13).
(E) The preventive effect of phytol is dependent on route of administration. Preventive effect of phytol on PIA in DA.Ncf1DA rats after IP (200 μl), SC (200 μl), or intranasal (25 μl) administration on days −5 and +5 (n = 7).
*p < 0.05, **p < 0.01, ***p < 0.001.