Macrophages Inhibit Neovascularization in a Murine Model of Age-Related Macular Degeneration
Figure 4
Injection of Macrophages Inhibits CNV
Cells obtained from bone marrow (BM) cultures (A) or spleen (B) were purified by magnetic beads and injected into the vitreous cavity on the day of laser treatment. Seven days later, the volume of the neovascular complex was determined by confocal microscopy. Asterisks indicate values significantly different from control; p-values given in parentheses are based on Student's t test.
(A) Volume of the neovascular complex for injection of PBS (14,470.9 ± 1,636.4 μm3), CD11b cells—1 × 105 cells (2,431.2 ± 551.3 μm3), and CD11b cells—5 × 105 cells (5,274.2 ± 772.5 μm3).
(B) Volume of the neovascular complex for injection of PBS (12,857.9 ± 1,094.9 μm3), CD3 cells (14,960.5 ± 2,502.0 μm3), CD11b cells (4,135.1 ± 714.4 μm3), and CD11c cells (15,063.3 ± 1,982.4 μm3).