Marked Differences in Human Melanoma Antigen-Specific T Cell Responsiveness after Vaccination Using a Functional Microarray
Figure 6
Differences in Functional Profiles between Three Patients with Different Clinical Outcomes
gp100 209–2M spots co-spotted with anti-IFNγ, anti-TNFα, anti-IL1b, and anti-IL-6 are shown for three separate patient samples. Patient 10735, who remains free of disease at this writing, displays strong IFNγ, TNFα, IL-1b, and IL-6 secretion. Patients 10710 and 10737 show strong IFNγ secretion, but weak to no TNFα, IL-1b, and IL-6 secretion; both patients experienced disease recurrence soon after these samples were drawn. Note the diffuse pattern of IL-1b and IL-6 capture, which differs from the focal capture of IFNγ and TNFα.