Snoo and Dpp Act as Spatial and Temporal Regulators Respectively of Adult Progenitor Cells in the Drosophila Trachea
Fig 1
Snoo effects DAP cell behaviour downstream of Fzr.
A)Wild type expression of Hdc. B)Ectopic expression of Hdc following forced tracheal over expression of snoo using btl-gal4 during L3. C)Loss of Hdc expression following RNAi mediated knockdown of snoo in the trachea via activation of btl-gal4 during L3. D)Expression of Hdc is lost when snoo is coexpressed with fzr in the trachea via btl-gal4 during L3. E)Expression of snoo as visualized by snoo-lacZ. F)Accumulation of Snoo::GFP following a pulse of tracheal expression (see methods). Red shows higher levels while blue/purple shows lower levels. G)Magnification of Tr2 and Tr3 showing nuclear accumulation of Snoo::GFP in DAP cells. H)Accumulation of Snoo::GFP when btl-gal4 is active continuously. I)Accumulation of Snoo::GFP when coexpressed with fzr. J)Wild type expression of Stg::GFP. K)Inactivation of stg expression after RNAi mediated knockdown of snoo in the trachea during L3. L)Ectopic Stg::GFP accumulation following btl-gal4 mediated tracheal overexpression of snoo during L3. Scale bars indicate 100um.