Genetic Interactions Involving Five or More Genes Contribute to a Complex Trait in Yeast
Figure 4
Segregation analysis of causal genes and identification of loci that complement END33S.
Spores from dissected tetrads were phenotyped and then genotyped at END3, FLO8, IRA2, MSS11, and TRR1. Results from the BY and 3S backcrosses are in A and B, respectively. In C, we show genotyping results for candidate loci on Chromosomes VII, XI, XII, and XV in a population of END33S segregants from a second-generation backcross. Lastly, in D, we show the different genotypes in the BY×3S cross that specify rough morphology. In A–D, blue and orange denote BY and 3S alleles, respectively, while grey in D indicates that either allele can occur. All rough individuals incorporated into this figure possessed IRA23S-Δ2933. The mutant allele is denoted in D by the label ‘mut’. Rough and smooth morphology are specified in A–C by black and light grey, respectively.