Retinoid-X-Receptors (α/β) in Melanocytes Modulate Innate Immune Responses and Differentially Regulate Cell Survival following UV Irradiation
Figure 4
Generation of Rxrα/Rxrβ double knockdown murine melanocytes.
(A) Primary murine melanocytes were transfected using a mixture of two shRNA-expressing plasmids targeting either Rxrα or Rxrβ. The Rxrα shRNA plasmid contains a GFP marker gene; Rxrβ shRNA contains a RFP marker gene. Cells positive for both GFP and RFP were sorted using FACS and re-cultured. (B) Re-cultured melanocytes following FACS express both GFP and RFP, indicating expression of both Rxrα and Rxrβ shRNA constructs. (C) RT-qPCR for measurement of Rxrα/β mRNA expression **** = p≤0.0001. (D) Western Blot for protein expression of RXRα in FACS-sorted cells.