RBPJ, the Major Transcriptional Effector of Notch Signaling, Remains Associated with Chromatin throughout Mitosis, Suggesting a Role in Mitotic Bookmarking
Figure 1
RBPJ associates with mitotic chromatin through direct DNA contacts.
Fluorescence microscopy imaging of live F9 cells transiently expressing eGFP-tagged RBPJ or RBPJ derivatives harboring mutations in residues predicted to contact DNA (green). Chromatin was counterstained with Hoechst 33342 (blue). (A) F9 cells expressing eGFP-RBPJ. (B) F9 cells expressing eGFP-RBPJK153A,S182A. (C) F9 cells expressing eGFP-RBPJR179A,R181A. (D) F9 cells expressing eGFP-RBPJR52A,K153A,S182A. R52A, K153 and S182 are conserved residues predicted to contact DNA bases. R179 and R181 are conserved residues predicted to contact the phosphodiester backbone.