Species and Population Level Molecular Profiling Reveals Cryptic Recombination and Emergent Asymmetry in the Dimorphic Mating Locus of C. reinhardtii
Figure 4
Expression patterns of mating locus genes.
Panels A–F show expression values from quantitative RT-PCR (qRT-PCR) experiments for indicated genes calculated as described in Materials and Methods. Each panel groups genes by their overall expression pattern as follows: A, MT+ gametic; B, MT− gametic; C, MT− only; D, early zygotic; E, zygotic; F, reduced in zygotes. RNA samples were derived from MT+ vegetative cells (PV) and gametes (PG), MT− vegetative cells (MV) and gametes (MG), and from zygotes at 10 minutes, 30 minutes, 1 hour, 2 hours and 3 hours after mating (Z10, Z30, Z1h, Z2h and Z3h respectively). Panels G, H show gels from semi-quantitative RT-PCR experiments in which G. MTA4 cDNA or H. internal control 18S ribosomal cDNA were amplified. * No expression detected.