Stepwise Activation of the ATR Signaling Pathway upon Increasing Replication Stress Impacts Fragile Site Integrity
Figure 6
ATR depletion compromises genome stability.
(A) Quantification of metaphases (M) with breaks in cells transfected as above and treated for 16 h with the indicated aphidicolin concentrations. Mean ± s.e.m are presented. (B) Correlations between replication fork speed and chromosome instability. The data presented in A and Figure 5C were used to plot the percentage of metaphase plates with chromosome breaks against fork speed in each condition of transfection and treatment. (C) Breaks occurring at FRA3B quantified as in C. (D) Correlations between replication speed and FRA3B instability evaluated as in B.