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Cell Polarity and Patterning by PIN Trafficking through Early Endosomal Compartments in Arabidopsis thaliana

Figure 2

BEN2 encodes a SM protein AtVPS45.

(A) Alignment of VPS45 protein sequences from various organisms. At: Arabidopsis thaliana, (At1g77140); Pp: Physcomitrella patens (XP_001758633); Dd: Dictyostelium discoideum (XP_635835); Sc: Saccharomyces cerevisiae (NP_011420); Hs: Homo sapiens (NP_009190). (B) Complementation by VPS45-GFP of ben2 mutant phenotypes. After BFA treatment (25 µM for 2 h), PIN1 accumulated in intracellular compartments in wild type root vascular tissue. Whereas intracellular accumulation of PIN1 was less pronounced in ben2 mutant, clear accumulation of PIN1 was detected in ben2 mutant harboring VPS45-GFP. Similarly, PIN2 accumulation in root epidermal cells upon BFA treatment was recovered in ben2; VPS45-GFP. Scale bar: 10 µm.

Figure 2

doi: https://doi.org/10.1371/journal.pgen.1003540.g002