Conditional Inactivation of the DNA Damage Response Gene Hus1 in Mouse Testis Reveals Separable Roles for Components of the RAD9-RAD1-HUS1 Complex in Meiotic Chromosome Maintenance
Figure 4
RAD51 foci persist in late pachytene and diplotene spermatocytes in the absence of Hus1.
A–E. Immunofluorescence staining for RAD51/DMC1 from zygotene through diplotene stage of meiosis in control (Cre-negative Hus1flox/Δ1) and Stra8-Cre Hus1 CKO males. Arrows indicate persistent foci, and asterisks indicate symmetrical foci located on either side of chromosomal cores. F–I. Quantification of RAD51 foci at the indicated stages. ** indicates p<0.01; * indicates p<0.05.