Dynamic Large-Scale Chromosomal Rearrangements Fuel Rapid Adaptation in Yeast Populations
Figure 1
A subset of EC diploid strains is highly tolerant to copper.
(A) Strains EC9, 10, 35, 36, 39, 57 and 58 can tolerate a high level of copper (10 mM CuSO4). Diploid EC strains were grown in YPD media overnight, serially diluted and plated on YPD plates containing different concentrations of CuSO4. Plates were incubated at 28°C until obvious colonies were formed. (B) Pulsed-field gel electrophoresis analysis reveals that the EC strains comprised three major karyotypes: EC cluster 1 (EC-C1) containing seven strains (EC9, 10, 35, 36, 39, 57 and 58), EC cluster 2 (EC-C2) containing six strains (EC13, 33, 34, 45, 59 and 60), and EC cluster 3 (EC-C3) containing only one strain (EC63). All copper-tolerant strains belong to EC-C1. M, yeast chromosomal DNA from a standard laboratory strain.