Systematic Identification of Rhythmic Genes Reveals camk1gb as a New Element in the Circadian Clockwork
Figure 4
The effect of camk1gb knockdown on larval locomotor activity rhythms.
A) Average locomotor activity of 6–8 dpf larvae injected with either control morpholino (black trendline), camk1gb morpholino (gray trendline) or co-injected camk1gb morpholino together with camk1gb mRNA (green trendline), under constant dim-light. Activity was measured as the average distance moved for time units of 10 min and smoothed using moving average. Error bars represent SE (n = 16). Black and gray horizontal boxes represent subjective night and day, respectively. B) Cumulative distribution of g-factor values for each group. Significant differences in the g-factor distribution were revealed between the control morpholino and camk1gb morpholino treated groups as well as between the rescue and camk1gb morpholino injected groups (Kolmogorov-Smirnov test, P-value<0.05 and <0.01, respectively). C) Representative activity profiles of control morpholino (black curve) and camk1gb morpholino (gray curve) injected larvae which correspond to the median g-factor of each group. The median g-factor values are marked by red circles in (D). D) Significant differences in the cumulative distribution of g-factor values between control morpholino (black curve) and camk1gb morpholino (gray curve) injected groups (n = 75), generated from the analysis of four similar experiments (Kolmogorov-Smirnov test, P-value<10−5).