Chemical Genetics Reveals a Specific Requirement for Cdk2 Activity in the DNA Damage Response and Identifies Nbs1 as a Cdk2 Substrate in Human Cells
Figure 1
Cdk2 activity is required for normal DNA damage resistance.
(A) Transient treatment with 10 µM 3-MB-PP1 for 48 hr does not affect efficiency of colony formation by wild-type or Cdk2as/as RPE-hTERT cells. (B) Treatment with 10 µM 3-MB-PP1 before and after irradiation (24 hr each) sensitizes Cdk2as/as RPE-hTERT cells to killing by IR. (C) Pre-treatment with 3-MB-PP1 for 24 hr does not sensitize Cdk2as/as cells to IR. (D) Treatment with 3-MB-PP1 for 24 hr after IR sensitizes Cdk2as/as cells to killing. Error bars are +/− standard deviation (SD) of duplicates.