Genetics and Regulatory Impact of Alternative Polyadenylation in Human B-Lymphoblastoid Cells
Figure 7
Genetic variation in IRF5 alternative polyadenylation and recovery of steady-state expression after induction.
Each data point represents IRF5 expression in B-lymphoblastoid cells measured by quantitative PCR after exposure to lipopolysaccharide (5 µg/mL). Each line style represents one 3′ length form of IRF5 mRNA. Each panel represents a cell line from a genetically distinct individual as indicated at top right, whose biallelic genotype at the proximal polyadenylation signal which encodes robust (short) or minimal (long) 3′ processing is shown above the schematic of the IRF5 gene; an inferred AU-rich element is indicated as a green box. The x-axis reports time in hours after treatment and the y-axis reports expression normalized to the maximum expression in GM10860. Error bars represent standard deviations (n = 2).