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The Burkholderia bcpAIOB Genes Define Unique Classes of Two-Partner Secretion and Contact Dependent Growth Inhibition Systems

Figure 3

Analysis of BcpA-HA.

Immunoblot of whole cell lysates of WT (E264BcpA-HA::pECG22), ΔbcpO (E264BcpA-HAΔbcpO::pECG22), ΔbcpB (E264BcpA-HAΔbcpB::pECG22), ΔbcpO::bcpO (E264BcpA-HAΔbcpO::bcpO::pECG22), ΔbcpB::bcpB (E264BcpA-HAΔbcpB::bcpB::pECG22), no tag (E264::pECG22) strains producing BcpA-HA were separated by SDS-PAGE and stained with anti-HA antibody.

Figure 3

doi: https://doi.org/10.1371/journal.pgen.1002877.g003