Pregnancy-Induced Noncoding RNA (PINC) Associates with Polycomb Repressive Complex 2 and Regulates Mammary Epithelial Differentiation
Figure 10
Microarray identifies potential targets of mPINC in HC11 cells.
(A) Heat map showing transcript profiling of biological replicates of control siNEG cells, siPINC1.0/1.6, and siPINC 5 days post-siRNA transfection ( p<0.01 and fold change >1.8 in either siPINC1.0/1.6 or siPINC relative to siNEG). (B) Graph indicating the most significantly enriched gene ontology terms in the mPINC knockdown data set. (C) Three heat map panels depicting genes that are differentially expressed between either undifferentiated (−LH) mPINC knockdown cells (left panel) or differentiated (+LH) mPINC knockdown cells (middle panel) and differentiated (+LH) mPINC overexpression cells (right panel). 181 genes were found to be differentially regulated by mPINC (141 genes are upregulated by knockdown and downregulated by overexpression, while 40 genes are downregulated by knockdown and upregulated by overexpression at a p<0.01 and fold change >1.4, both for the overexpression and either knockdown group in the opposite direction). Genes whose expression was validated by qPCR are indicated in the heat map on the left. (D) qPCR verified differential expression of genes in mPINC knockdown (KD) compared to overexpression (OE) cells. qPCR was performed on biological triplicates, normalized using Gapdh, and shown as fold change compared to the negative control (either siNEG or LeGO-GFP).