The Secreted Immunoglobulin Domain Proteins ZIG-5 and ZIG-8 Cooperate with L1CAM/SAX-7 to Maintain Nervous System Integrity
Figure 2
zig-5 and zig-8 affect axon positioning in the ventral nerve cord.
(A) PVQ axon flip-over (red arrowhead) in zig-5 zig-8 double mutants, observed first in later larval stages, but not earlier. Ventral view, anterior is to the left. Grey arrows indicate the location of axon flip-over. Scale bar is 5 µm. (B) Quantification of axon flip-over defects in zig-5 zig-8 double mutants, using transgenes oyIs14, hdIs29, bwIs2, oxIs12 and zdIs13, respectively. Single mutants zig-5 and zig-8 are wild type [11]. Animals were paralyzed with unc mutants or levamisole (Lev). Proportions of different animal populations were compared using the z-test. “*” indicates p<0.01.