Polyglutamine Toxicity Is Controlled by Prion Composition and Gene Dosage in Yeast
Figure 3
Effects of AQT on polyglutamine aggregation and Sup35 toxicity.
A and B – Typical aggregation patterns of 103Q (multiple dots, A) and 103QP (single clump, B) are not affected by AQT, as confirmed by fluorescence microscopy. C – AQT mutant ameliorates toxicity of excess Sup35 or Sup35N in the [PSI+] strain. Sup35 and Sup35N proteins were expressed from centromeric plasmid under control of the galactose-inducible promoter. Cells were grown on the -Ura/glucose medium selective for the plasmid for 1 day. Serial decimal dilutions were plated onto -Ura/Gal medium.