SMG-1 and mTORC1 Act Antagonistically to Regulate Response to Injury and Growth in Planarians
Figure 7
Rapamycin decreases neoblast proliferation and increases the survival rate in Smed-smg-1 RNAi animals.
A. Number of H3P positive cells per mm2 of un-cut planarians or 6 hR planarians that were previously treated with daily injections of DMSO, 20 nM rapamycin, 30 nM rapamycin or 40 nM rapamycin during 2 weeks. Error bars are s.d from the mean, the asterisk indicate P<0.05 while ns indicates “not significant” (P>0.05) using two-tailed Student's test with equal sample variance relative to the corresponding DMSO treated planarians. 6 hR shows an increased mitotic peak respect to the pre-cut condition despite the treatments (P<0.01). No significant differences (P>0.05) are observed either between the 30 nM and 40 nM rapamycin treatment in the pre-cut planarians or between the different rapamycin treatments in the 6 hR planarians. B. Number of H3P positive cells per mm2 in control RNAi and Smed-smg-1 RNAi planarians at 20 hR that were treated with daily injections of DMSO, 20 nM rapamycin, 30 nM rapamycin or 40 nM rapamycin. Outside the bars two asterisks (p<0.01) show significant differences while ns (p>0.05) shows no significant differences relative to the control RNAi animals. The asterisks inside the blue bars show significance relative to the 20 hR control RNAi worms DMSO treated whereas inside the red bars the significance is shown relative to the 20 hR Smed-smg-1 RNAi worms DMSO treated. Error bars are s.d from the mean and asterisks indicate P<0.05 (one asterisk) or P<0.01 (two asterisks) or P>0.05 (ns) using two-tailed Student's test with equal sample variance. ≥8 planarians per time point. C. Kaplan-Meier curves demonstrate increased survival of Smed-smg-1 RNAi animals when treated with 20 nM, 30 nM or 40 nM rapamycin. Four asterisks indicate p<0.0001 with log-rank analysis of survival curves. Representative images of surviving animals after Smed-smg-1 RNAi and treated with 40 nM rapamycin are shown at 45 dR. Scale bars indicate 300 µm.