Akirin Links Twist-Regulated Transcription with the Brahma Chromatin Remodeling Complex during Embryogenesis
Figure 7
Akirin and the BRM complex co-occupy the Dmef2 enhancer during embryogenesis.
Chromatin immunoprecipitations were performed on extracts prepared from 2–4, 4–6, and 6–10 hour-old yw (Blue bars) and akirin2 mutant (Red bars) embryos using anti-Twist (A), anti-Akirin (B), and anti-Moira (C) antibodies. The presence of the Dmef2 enhancer or even-skipped MHE enhancer (eve MHE) was assayed in precipitated DNA samples using quantitative PCR. Values for preimmune (stippled bars) and immunoprecipated samples (solid color bars) are presented as percent of total input fraction. Akirin and the core BRM subunit Moira both co-occupy the Dmef2 enhancer with Twist in 2–4 and 4–6 hour-old embryos. The occupancy of Moira at this enhancer appears to be Akirin-dependent, as Moira occupancy decreases by approximately 64% in akirin mutant extracts. (A–C) While Twist and Moira occupy the eve MHE enhancer, Akirin is not enriched at this region in wild-type or mutant extracts, and the absence of Akirin does not affect the occupancies of either Twist or Moira.