Identification of Evolutionarily Conserved Exons as Regulated Targets for the Splicing Activator Tra2β in Development
Figure 7
Point mutants in the Nasp-T exon within candidate Tra2β binding sites are still able to bind to Tra2β.
RNA-protein interactions were monitored by EMSAs. The average position of the slowest migrating complex in the lane containing 10 ng of added Tra2β protein is indicated by an asterisk, and the RNA probes used were as in Figure 4 but containing the appropriate point mutation.