Gamma-Tubulin Is Required for Bipolar Spindle Assembly and for Proper Kinetochore Microtubule Attachments during Prometaphase I in Drosophila Oocytes
Figure 3
Kinetochores fail to make direct contacts to kinetochore microtubules in γtub37C mutant oocytes.
Fixed prometaphase I oocytes were treated with antibodies against α-tubulin and CID, as well as the DNA dye DAPI. Selected regions are magnified in the insets. Insets show only CID and α-tubulin for better visualization of microtubule interactions with CID foci. (A) CID foci directly interact with kinetochore microtubules (arrows) in a wild-type oocyte. (B–D) CID foci show aberrant kinetochore microtubule attachments in γtub37CP162L mutant oocytes. Arrows point to examples of CID foci with no apparent direct microtubule interactions and the arrowhead points to an example of a CID focus with potentially a lateral microtubule interaction. (E–F) Shows spindles from γtub37C3/Df mutant oocytes. The arrows point to examples of CID foci lacking clear microtubule interactions and the arrowhead points to an example of a CID focus showing only a potential lateral interaction to microtubules. Images are single Z sections to highlight the interaction of CID foci with the microtubules. Black levels were adjusted to allow better visualization of CID foci. Scale bars are in microns.