Integrated Genome-Scale Prediction of Detrimental Mutations in Transcription Networks
Figure 1
Binding site conservation relates more to the importance of the regulator than the target gene.
The conservation of 19,671 experimentally determined binding sites for 119 different TFs was evaluated among 36 different strains of S. cerevisiae (within species conservation) and among 3 additional sensu stricto Saccharomyces species (between species conservation). Conservation within species implies functional conservation within all strains. Conservation between species implies functional conservation in at least 2 out of the 3 additional species. If at least one binding site for a particular transcription factor is conserved the regulatory interaction is considered as conserved. Transcription factor (TF) binding sites (A) and regulatory interactions (B) targeting genes required for growth tend to be slightly more conserved within and between species. (C) Binding sites for essential TFs are more conserved within and between species, also when controlling for essentiality of the target gene (D), this association is stronger than for the target genes (compare A and C). P-values calculated by chi square test; OR = odds ratio; error bars show 95% confidence intervals calculated assuming a binomial distribution and using the Wilson score interval. The number of binding sites considered is shown below each bar.