Evolution Meets Disease: Penetrance and Functional Epistasis of Mitochondrial tRNA Mutations
Figure 5
Metabolic labeling of the assembled OXPHOS complexes.
A) Fluorogram, after BNGE, of the mitochondrial translation products of mutant and control cells, pulse-labeled with [35S]-methionine for 2 hr in the presence of cycloheximide (P) and chased (C) for 48 hours; CI-CV, complexes I to V. B) Fluorograms of two-dimensional electrophoresis (BNGE followed by SDS-PAGE), of the mitochondrial translation products obtained in b (48h chase). I-V, indicate the position of complexes I to V. Asterisks show the presence of low molecular weight subcomplexes containing CYTB and COI in mutant cell lines.