Expression of Linear and Novel Circular Forms of an INK4/ARF-Associated Non-Coding RNA Correlates with Atherosclerosis Risk
Figure 1
Identification and characterization of ANRIL splice variants.
A, 3′ and 5′ RACE was performed using primers directed against exons 4 and 6 where long stretches of unique sequence were observed (top). The resulting PCR products were cloned and sequenced, revealing several novel exons shown in blue (10a and 13b) and multiple non-colinear species (red). B, Equal quantities of total RNA were harvested from growing cell lines of various tissue types and absolute expression of the indicated transcript was determined. Expression levels are shown in a box-whisker plot on a log10 scale in 11 of 27 analyzed cell lines which did not harbor homozygous 9p21 deletion. Validated Taqman detection strategies for the indicated ANRIL species are shown (top).