Caenorhabditis elegans SMA-10/LRIG Is a Conserved Transmembrane Protein that Enhances Bone Morphogenetic Protein Signaling
Figure 3
SMA-10 binds BMP receptors and enhances BMP signaling.
SMA-10 enhances BMP signaling in HepG2 cells (A). Cells were transfected with a BMP-responsive transcriptional reporter (I-BRE:luciferase) and either SMA-10 or a control vector. Cells were either left unstimulated (black bars) or were stimulated with 2.5 nM BMP2 (grey bars). The average of four transfected wells is shown plus and minus the standard deviation. SMA-10 binds SMA-6 and DAF-4 (B). 293T cells were co-transfected with FLAG-tagged SMA-10 and HA-tagged SMA-6 or DAF-4 receptors. Lysates were immunoprecipitated with anti-FLAG (SMA-10) and blotted for HA (SMA-6 or DAF-4). LRIG1 is able to interact with several mammalian BMP receptors (C). Cells were co-transfected with various HA-tagged BMP receptors and 3x FLAG-tagged LRIG1, then lysates were immunoprecipitated with anti-HA antibody and blotted with anti-FLAG to detect LRIG1.