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Peroxiredoxin Tsa1 Is the Key Peroxidase Suppressing Genome Instability and Protecting against Cell Death in Saccharomyces cerevisiae

Figure 2

Construction of the strains expressing desired genes under control of the TSA1 promoter.

The S. cerevisiae strains expressing TSA2 or mutated forms of TSA1 (tsa1C47S, tsa1C170S, and tsa1CCS) under control of the endogenous TSA1 promoter were constructed as described in Materials and Methods. The desired coding sequence was tailed by PCR with sequence homologous to regions flanking the chromosomal TSA1 to allow targeted recombination.

Figure 2

doi: https://doi.org/10.1371/journal.pgen.1000524.g002