MSH2 ATPase Domain Mutation Affects CTG•CAG Repeat Instability in Transgenic Mice
Figure 2
Somatic mosaicism in 8-month-old Msh2+/+, Msh2G674A/+ and Msh2G674A/G674A transgenic males.
bl, blood; p, pancreas; l, liver; t, testis; s, sperm; h, heart; b, brain; c, cerebellum; q, quadriceps. M. 250 bp DNA ladder converted to number of CTG repeat units in the given PCR product. Msh2 +/+/, m/+ and m/m represent genotypes at the murine locus for Msh2. +: wild-type allele; m: Msh2G674A mutation. The Msh2 +/+, m/+ and m/m males in tail extracted DNA at weaning contained 525, 516 and 500 CTG repeats, respectively.