ERCC1/XPF Protects Short Telomeres from Homologous Recombination in Arabidopsis thaliana
Figure 3
Fluorescence in situ hybridisation analysis (FISH) of the structure and origin of dicentric chromosome bridges.
A: Examples of FISH analysis of mitoses from flower pistils: from right to left, fluorescent probes to telomeric repeat DNA (green), pooled 10 subtelomeric BACs (red), DAPI-stained DNA (white) and merged image. From top to bottom: examples of anaphase bridges of class I (both subtelomeric and telomeric foci in bridge), class II (only subtelomeric foci present in the bridge) and class III (bridges with neither subtelomeric nor telomeric foci). The scale bar represents 5 µm. B: Distribution of the three classes of anaphase bridges in Attert (generations G5, G7) and Atercc1/Attert (G3, G5) mutant plants. Relative percentages and numbers counted in each class, mutant and generation are indicated in the bars.