Field-Caught Permethrin-Resistant Anopheles gambiae Overexpress CYP6P3, a P450 That Metabolises Pyrethroids
Figure 4
HPLC chromatograms of CYP6P3 reactions.
(A) and (B) show CYP6P3 reactions with 10 µM permethrin. (C) and (D) show CYP6P3 reactions with 10 µM deltamethrin. Substrate peaks for cis- and trans-permethrin stereoisomers and deltamethrin are indicated. (B) and (D) are overlaid traces of reactions quenched after 0 min (light trace) and 30 min (dark trace) showing substrate elimination in the presence of NADPH. (A) and (C) are overlaid negative control reactions quenched after 0 and 30 min in the absence of NADPH. Putative NADPH-dependant metabolite peaks are indicated by arrows.