E1 Ubiquitin-Activating Enzyme UBA-1 Plays Multiple Roles throughout C. elegans Development
Figure 3
A) Sperm localization in the hermaphrodite reproductive tract. Wild type and uba-1(it129) adults at 25°C were fixed and stained with DAPI to count sperm nuclei. Arrowheads, location of spermathecae; small arrows, sperm displaced into the uterus. B) Summary of sperm localization data. Shown are mean values and standard deviations per hermaphrodite (N = 6). T0, before egg-laying commences; T1, after 1–2 ovulations; T2, 8 h post-T1; T3, 8 h post-T2. C) In vitro activation. Spermatids from wild type and uba-1(it129) males at 25°C were activated with monensin. Arrow indicates pseudopod of crawling spermatozoon.