Regulation and Role of Arabidopsis CUL4-DDB1A-DDB2 in Maintaining Genome Integrity upon UV Stress
Figure 6
ATR-dependent DDB1A-GFP nuclear shuttling and DDB2 stability.
(A) DDB1A-GFP dynamics upon UV-C exposure (600 J/m2) in WT (upper panel) and in atr-2 mutant plant (lower panel). Bar = 75 µm. Pictures are representative of 3 different experiments using 2 independent transgenic lines. (B) Higher magnification of DDB1A-GFP localisation 15 min upon UV-C exposure in either ddb1a-2 or atr-2 mutant backgrounds. Chromatin is stained by DAPI (blue). (C) Immunoblot revealing DDB2 content upon UV-C exposure (900 J/m2) in WT, cul4-1, ddb1a-2 and atr-2 Arabidopsis plants. Coomassie blue staining was used as loading control.