Leukocyte Tyrosine Kinase Functions in Pigment Cell Development
Figure 4
shd mutants show elevated NCC apoptosis, but pigment cell numbers are not recipricolly elevated.
A) Graph shows mean±s.d. fragmenting GFP+ NCCs in trunk and tail of embryos from an incross of 7.2sox10;egfp, shdty82/− carriers. Embryos were sorted at 30–48 hpf for dying premigratory or medial pathway NCCs, then genotyped at c. 3 dpf by iridophore phenotype. Two-tailed t test shows highly significant differences (P<0.0001). B–D) Melanophore number (mean±s.d.) in trunk and tail dorsal stripe at 3 dpf (B) and total dct-positive melanoblast number in posterior trunk and tail (C) and gch-positive xanthoblast number on lateral pathway of posterior trunk and tail in one side (D) at 30 hpf are indistinguishable in shd mutants and WT siblings. Two-tailed t test shows no difference in all cases (p>0.05).