Network Discovery Pipeline Elucidates Conserved Time-of-Day–Specific cis-Regulatory Modules
Figure 6
Thermocycles and Photocycles Have Distinct Phase Relationships
(A) Long day photocycles (16 h light/8 h dark) phase delay genes compared to short day photocycles (8 h light/16 h dark). Expression pattern is the average of 23 genes displaying a 6-h phase delay between long day (black, phase 13 h), and short day (red, phase 7 h).
(B) Long day photocycles globally phase delay genes as compared to short day photocycles. Phase shift topology graph plots percent of genes phase shifted per phase bin (y-axis) by the reference condition phase (x-axis). Only genes that are rhythmic between both conditions are used in this analysis. Percent of genes was calculated as the number of genes with a given phase shift per phase divided by the total number of genes with that phase. A positive phase shift reflects a later phase than the reference condition, and a negative phase shift reflects an earlier phase than the reference condition. Long day photocycle is the reference condition and consistent with (A), long day photocycles delay the phase (positive phase shift).
(C) Phase-shift topology between LLHC and LDHH_ST, where LLHC is the reference phase.
(D) Phase-shift topology between LL_LLHH-SH and LDHH_ST, where LL_LDHH-SH is the reference phase.
(E) Phase-shift topology between LLHC and LDHC, where LLHC is the reference phase.
(F) Phase-shift topology between LL_LLHC and LLHC, where LL_LLHC is the reference phase.