A Screen for Suppressors of Gross Chromosomal Rearrangements Identifies a Conserved Role for PLP in Preventing DNA Lesions
Figure 7
Inhibition of Pdxk Causes DSBs in Human Cells
(A) HeLa cells display elevated levels of 53BP1 foci following inhibition of Pdxk by 4-DP.
(B) Quantitation of cells with 53BP1 foci following 4-DP treatment.
(C) 4-DP treatment activates the DNA damage checkpoint in HeLa cells as measured by Chk2 phospho-Thr68 and γ-H2AX immunoblotting.
(D) SiHa cells treated with 4-DP accumulate γ-H2AX in S-phase, as measured by flow cytometry.