Discovery and Analysis of Evolutionarily Conserved Intronic Splicing Regulatory Elements
Figure 6
Enrichment and Depletion of ISREs Proximal to Alternative Exons Differentially Included or Skipped in Brain or Muscle from Splicing-Specific Microarray Data
Intronic regions upstream and downstream of exons predicted to be included/skipped at different significance levels (see Protocol S1 for details on the dataset). The significance of enrichment (red boxes)/depletion (green boxes) are reported as log10 p values; a plus sign indicates enrichment, and a minus sign indicates depletion. For example, an ISRE represented by GCATG was enriched in intronic regions downstream of brain-included exons at p < 1 × 10−4 (reported as 4). The motifs highlighted in yellow have been experimentally verified to alter splice site choice in our reporter constructs.
(A) Downstream ISREs enriched/depleted in the downstream intronic regions.
(B) Upstream ISREs enriched/depleted in the upstream intronic regions.