The Role of DNA Double-Strand Breaks in Spontaneous Homologous Recombination in S. cerevisiae
Figure 7
Rad51-CFP and Rad52-C180A-YFP Are Recruited to a Specific DNA DSB
(A) Assay in Chromosome V for visualizing an I-SceI-endonuclease inducible DSB. Red boxes: tetO sites. Cyan triangle: I-SceI cut-site (I-SceIcs). Solid circle: centromere.
(B) Localization of Rad51-CFP and Rad52-C180A-YFP foci to an I-SceI-endonuclease-induced DSB. The panels show YFP, CFP, RFP, RFP/YFP-merged, and CFP/RFP/YFP-merged, as well as a bright field image of representative cells containing Rad51-CFP and Rad52-C180A-YFP in a strain with a tetO tandem array next to an I-SceIcs on Chromosome V. The tetO tandem array is visualized by TetI-RFP as a red focus. The Rad51-CFP, Rad52-YFP, and TetI-RFP foci are marked by arrowheads. Scale bar, 3 μm.
(C) Quantitative analysis of co-localization between Rad52-C180A-YFP/RFP–TetI foci and Rad51-CFP foci. The wild-type dataset is shown for comparison.