Oriented Scanning Is the Leading Mechanism Underlying 5′ Splice Site Selection in Mammals
Figure 8
Role of G Triplets in IVS7 Splice Site Recognition
Top: Wild-type (middle line) and mutated (lower and upper lines) 5′ pseudo-sites in the F7 minigene. Dots show the location of the introduced mutations. Suffixes of the constructs' names and the resulting CVs are indicated. Middle: Schematic representation of the constructs transfected in CHO cells. Mutated splice sites are represented by a hatched box (see legend to Figure 2A for the meaning of each symbol). Bottom: RT-PCR amplification of F7 transcripts isolated from CHO cells transfected with the above-mentioned constructs. The products corresponding to the different PCR fragments, as identified by sequencing, are schematically represented on both sides of the figure.