Implementation of Complex Biological Logic Circuits Using Spatially Distributed Multicellular Consortia
Fig 4
Design and in vivo implementation of a 4-input comparator.
(A) Schematic representation and spatial distribution of the cells used in the 2 bit magnitude comparator. (B) Truth table (left), microscope images (middle) and percentage of FACS fluorescent-positive cells (right). Cells were mixed proportionally and combinations of four inputs (17-β-estradiol (EST), a1; doxycycline (DOX), a0; progesterone (PRO), b1; and dexamethasone (DEX), b0) were added simultaneously to the six chambers. After computing, for each combination of inputs, the percentage of GFP or mCherry positive cells from the corresponding three chambers were analyzed using FACS. Green (GFP) and red (mCherry, (mCh) bars represent output quantification (A>B red; A<B green; A = B no signal). Data represent the mean and standard error of the chamber with highest % of positive cells from three independent experiments.