Spatio-temporal Dynamics and Mechanisms of Stress Granule Assembly
Fig 6
Analysis of the dynamics of SG assembly.
(A) Time courses of SG, TIA1, TIA2, and TIA3 are shown. TIA1 decreased monotonically as the increase in the number of SG. TIA2 and TIA3 increased quickly just after the beginning of SS, and soon they decreased. The peak number of TIA2 was small, and that of TIA3 was 9, which was smaller than the peak number of SG. (B) The decrease in the number of SG after the peak was caused by the fusion between SGs. If we aligned the occurrences of fusions (black pulsatile vertical lines) to the time course of the number of SGs (red line), the occurrences of the decrement in the time course and those of fusion events coincided perfectly. Two SGs at 3200 sec (white arrowheads in the bottom left panel) fused together forming one larger SG at 3210 sec (white arrowhead in the bottom right panel). (C) SG distribution at 60 min was changed by the change in pm/pn. Small change in pm/pn changed the SG distribution.